Tetrahedral DNA conjugates from pentaerythritol-based polyazides. Issue 19 (12th May 2016)

• Record Type: Journal Article
• Authors: Ponomarenko, Anna I.; Brylev, Vladimir A.; Sapozhnikova, Ksenia A.; Ustinov, Alexey V.; Prokhorenko, Igor A.; Zatsepin, Timofei S.; Korshun, Vladimir A.
• Is Part Of: Tetrahedron. Volume 72:Issue 19(2016)
• Page Start: 2386
• Page End: 2391
• Publication Date: 2016-05-12
• Subjects: Oligonucleotides -- Conjugates -- Branched DNA -- Click reaction -- Fluorescent dyes; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2016.03.051
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: Branching points in DNA nanostructures are usually 3- or 4-way junctions maintained by Watson-Crick non-covalent interactions. However, covalently bound DNA stars could improve the diversity, strength and integrity of DNA nanoscale constructions. We report here the convenient synthesis of 3- and 4-fold pentaerythritol-based azides and their use for the assembly of branched conjugates containing the same or different oligonucleotides (ODNs) and/or fluorescent dyes by stoichiometry controlled copper (I) catalyzed azide alkyne cycloaddition (CuAAC) functionalization. Graphical abstract: