C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice. (March 2016)

• Record Type: Journal Article
• Authors: Sun, Aning; Ren, Gaiyan; Deng, Chao; Zhang, Jingjing; Luo, Xiaoping; Wu, Xiaojun; Mani, Sridhar; Dou, Wei; Wang, Zhengtao
• Is Part Of: Journal of functional foods. Volume 21(2016)
• Page Start: 418
• Page End: 430
• Publication Date: 2016-03
• Subjects: COX-2 cyclooxygenase 2 -- CRC colorectal cancer -- DSS dextran sodium sulphate -- ELISA enzyme-linked immunosorbent assay -- ERK extracellular signal-regulated kinase -- HPLC high pressure liquid chromatography -- IBD inflammatory bowel disease -- IKK IκB kinase -- IL interleukin -- IκBα inhibitor kappa B-α -- iNOS inducible nitric oxide synthase -- IRAK1/2/4 interleukin-1 receptor-associated kinase 1/2/4 -- JNK c–Jun N–terminal kinase -- LPS lipopolysaccharide -- MAPK mitogen-activated protein kinase -- MCP-1 monocyte chemotactic protein-1 -- MPO myeloperoxidase -- MyD88 myeloid differentiation factor 88 -- NF-kB nuclear factor kappa B -- ORI orientin -- PPRs pattern recognition receptors -- qPCR quantitative polymerase chain reaction -- ROS reactive oxygen species -- siRNA small interfering RNA -- TLRs toll-like receptors -- TNF-α tumour necrosis factor-α -- TRAF-6 TNF receptor-associated factor-6 -- UC ulcerative colitis; DSS-induced colitis -- TLR4 -- NF-kB -- MAPK -- Orientin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.12.029
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Orientin attenuated DSS-induced colitis in mice. Orientin decreased the production of inflammatory mediators. Orientin down-regulated the DSS-induced expression of TLR4. Orientin inhibited the DSS-induced activation of NF-κB signalling. Orientin inhibited the DSS-induced activation of MAPK signalling molecules. Abstract: Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-4 after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.