Piceatannol protects ARPE-19 cells against vitamin A dimer-mediated photo-oxidative damage through activation of Nrf2/NQO1 signalling. (October 2016)

• Record Type: Journal Article
• Authors: Lu, Bing; Sun, Tao; Li, Weinan; Sun, Xiaodong; Yao, Xiaomin; Sun, Xiangjun
• Is Part Of: Journal of functional foods. Volume 26(2016)
• Page Start: 739
• Page End: 749
• Publication Date: 2016-10
• Subjects: Piceatannol -- Oxidative stress -- Nrf2 pathway -- Retinal pigment epithelium (RPE) cells -- Vitamin A dimer; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.08.046
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Piceatannol protects ARPE-19 cells against apoptosis induced by photo-oxidation. The protective effect of piceatannol is due to activating the Nrf2/NQO1 pathway. PI3K/Akt may be a chief pathway involved in Nrf2/NQO-1 induction by piceatannol. Abstract: Piceatannol, abundant in grapes and red wine, may prevent photo-oxidative damage to the eyes. During the aging process, dimers of dietary vitamin A accumulate in retinal pigment epithelium (RPE) cells and photo-oxidation of these dimers causes RPE death. This death is associated with age-related retinal degenerative disease and blindness. In this study, we investigated the underlying mechanism of photo-oxidation-induced apoptotic inhibition using piceatannol. To mimic Vitamin A dimer-mediated photo-oxidation, ARPE-19 cells with accumulated vitamin A dimers were used as a model to study apoptosis. Cells were either pretreated with piceatannol or untreated and exposed to blue light-induced endoplasmic reticulum stress (ER stress). Apoptosis was then measured and piceatannol was noted to inhibit apoptosis and ER stress. The mechanism by which this protective effect of piceatannol occurs is through the nuclear factor erythroid-derived 2-related factor 2 (Nrf2)/NAD(P)H quinine oxidoreductase 1(NQO1) pathway, which is likely due to activation of Akt signalling.