Antimicrobial activity and differentiation of anthocyanin profiles of rabbiteye and highbush blueberries using HPLC–DAD–ESI-MSn and multivariate analysis. (October 2016)

• Record Type: Journal Article
• Authors: Pertuzatti, Paula Becker; Barcia, Milene Teixeira; Rebello, Ligia Portugal Gomes; Gómez-Alonso, Sergio; Duarte, Renata Maria Teixeira; Duarte, Marta Cristina Teixeira; Godoy, Helena Teixeira; Hermosín-Gutiérrez, Isidro
• Is Part Of: Journal of functional foods. Volume 26(2016)
• Page Start: 506
• Page End: 516
• Publication Date: 2016-10
• Subjects: Vaccinium ashei Reade -- Vaccinium virgatum Aiton -- Vaccinium corymbosum L. -- Pathogenic bacteria -- Natural antimicrobial -- LCMS; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.07.026
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: All blueberry cultivars presented anti- Escherichia coli activity. Blueberry anthocyanins comprise the series of six glycosides of five anthocyanidins. Two complete acetylated series and some minor malonylated anthocyanins can be found. At least four anthocyanin profiles were differentiated in the analysed blueberries. The occurrence of acetylated anthocyanins characterizes most highbush cultivars. Abstract: This study aims to perform in vitro analyses of the antimicrobial activity of ten blueberry cultivars, to characterize the anthocyanin composition of these fruits, and to use multivariate analysis to compare the results. All blueberry cultivars presented anti- Escherichia coli activity. Furthermore, the complete series of six glycosides (galactoside, glucoside, arabinoside, xyloside, acetylgalactoside and acetylglucoside) of five anthocyanidins (delphinidin, cyanidin, petunidin, peonidin, and malvidin) were detected, together with six malonyl derivatives of the main anthocyanins. The rabbiteye cultivars showed higher proportions of cyanidin 3-galactoside than the highbush cultivars, the latter accounting for higher proportions of the 3-arabinosides of delphinidin and malvidin. The total anthocyanin content was between 10.8 and 26.5 g/kg of dry weight, and the concentration depended on the size of the berries, with the smaller rabbiteye cultivars exhibiting higher levels of anthocyanins.