Identification of major Toxoneuron nigriceps venom proteins using an integrated transcriptomic/proteomic approach. (September 2016)

• Record Type: Journal Article
• Authors: Laurino, Simona; Grossi, Gerarda; Pucci, Pietro; Flagiello, Angela; Bufo, Sabino Aurelio; Bianco, Giuliana; Salvia, Rosanna; Vinson, S. Bradleigh; Vogel, Heiko; Falabella, Patrizia
• Is Part Of: Insect biochemistry and molecular biology. Volume 76(2016:Sep.)
• Page Start: 49
• Page End: 61
• Publication Date: 2016-09
• Subjects: Venom -- Hymenoptera -- Venom glands -- Endoparasitoids -- Gene Ontology; HPLC-MS/MS high performance liquid chromatography tandem mass spectrometry -- 2DE two-dimensional gel electrophoresis -- PDV polydnavirus -- TnBV Toxoneuron nigriceps Bracovirus -- PBS Phosphate buffered saline solution -- GO Gene Ontology -- IEF first dimension isoelectric focusing -- PO polyphenol oxidase -- HS heparan sulfate -- ECM extracellular matrix; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2016.07.001
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: Endoparasitoids in the order Hymenoptera are natural enemies of several herbivorous insect pest species. During oviposition they inject a mixture of factors, which include venom, into the host, ensuring the successful parasitism and the development of their progeny. Although these parasitoid factors are known to be responsible for host manipulation, such as immune system suppression, little is known about both identity and function of the majority of their venom components. To identify the major proteins of Toxoneuron nigriceps (Hymenoptera: Braconidae) venom, we used an integrated transcriptomic and proteomic approach. The tandem-mass spectrometric (LC-MS/MS) data combined with T . nigriceps venom gland transcriptome used as a reference database resulted in the identification of a total of thirty one different proteins. While some of the identified proteins have been described in venom from several parasitoids, others were identified for the first time. Among the identified proteins, hydrolases constituted the most abundant family followed by transferases, oxidoreductases, ligases, lyases and isomerases. The hydrolases identified in the T . nigriceps venom glands included proteases, peptidases and glycosidases, reported as common components of venom from several parasitoid species. Taken together, the identified proteins included factors that could potentially inhibit the host immune system, manipulate host physiological processes and host development, as well as provide nutrients to the parasitoid progeny, degrading host tissues by specific hydrolytic enzymes. The venom decoding provides us with information about the identity of candidate venom factors which could contribute to the success of parasitism, together with other maternal and embryonic factors. Graphical abstract: Highlights: Thirty one proteins were identified through an integrated transcriptomic and proteomic approach in the parasitoid wasp venom. Transcript abundance correlated with higher intensity of spots on 2DE protein gels. Hydrolases were the most abundant venom proteins. Identified enzymes are likely to inhibit host immunity and to degrade host tissues.