Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Issue 4 (August 2016)

• Record Type: Journal Article
• Authors: Agmon, Gillie; Christman, Karen L.
• Is Part Of: Current opinion in solid state & materials science. Volume 20:Issue 4(2016)
• Page Start: 193
• Page End: 201
• Publication Date: 2016-08
• Subjects: ASC adipose derived stem cell -- ECM extracellular matrix -- EDC 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide -- ESC embryonic stem cell -- hASC human adipose derived stem cell -- hESC human embryonic stem cell -- hMSC human mesenchymal stem cell -- iPSC induced pluripotent stem cell -- mESC murine embryonic stem cell -- MSC mesenchymal stem cell -- NHS N-hydroxysulfosuccinimide -- NSC neural stem cell -- PDMS polydimethylsiloxane; Decellularization -- Differentiation -- Extracellular matrix -- Tissue engineering -- Stem cell; Materials science -- Periodicals; Solid state physics -- Periodicals; 620.11
• DOI: 10.1016/j.cossms.2016.02.001
• Languages: English
• ISSNs: 1359-0286

Abstract

Highlights: Different methods of ECM material preparation provide various benefits. ECM of each tissue has a unique architecture and biochemical composition. ECM materials have tissue specific effects that can direct cell differentiation. Decellularized tissues are a promising platform for tissue engineering applications. Abstract: Decellularized tissues have become a common regenerative medicine platform with multiple materials being researched in academic laboratories, tested in animal studies, and used clinically. Ideally, when a tissue is decellularized the native cell niche is maintained with many of the structural and biochemical cues that naturally interact with the cells of that particular tissue. This makes decellularized tissue materials an excellent platform for providing cells with the signals needed to initiate and maintain differentiation into tissue-specific lineages. The extracellular matrix (ECM) that remains after the decellularization process contains the components of a tissue specific microenvironment that is not possible to create synthetically. The ECM of each tissue has a different composition and structure and therefore has unique properties and potential for affecting cell behavior. This review describes the common methods for preparing decellularized tissue materials and the effects that decellularized materials from different tissues have on cell phenotype.