MicroRNA-155 Deficiency in Kupffer Cells Ameliorates Liver Ischemia-Reperfusion Injury in Mice. Issue 7 (July 2017)

• Record Type: Journal Article
• Authors: Li, Yakun; Ma, Dongxia; Wang, Zhimin; Yang, Jun
• Is Part Of: Transplantation. Volume 101:Issue 7(2017)
• Publication Date: 2017-07
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; Transplantation immunology -- Periodicals; 617.95
• DOI: 10.1097/TP.0000000000001765
• Languages: English
• ISSNs: 0041-1337

Abstract

Abstract : Background: MicroRNA-155 (miR-155) is known to be involved in autoimmune diseases, inflammation, and transplantation. However, its role in a warm hepatic ischemia-reperfusion (IR) model has not been fully elucidated. Methods: Partial hepatic IR was performed in wild-type and miR-155–deficient mice treated with or without GdCl3, and then the serum transaminase concentration and histology were analyzed. Kupffer cells (KCs) were isolated from the liver after IR, and immunohistochemistry was used to evaluate activation and polarization. In addition, the mRNA concentrations of various inflammatory cytokines were measured. Macrophages were obtained from the abdominal cavity and challenged with or without lipopolysaccharide to determine the influence of miR-155 deficiency on macrophage polarization in vitro. Furthermore, we used in vitro coculture assays to determine the effect of miR-155 deficiency on hepatocyte apoptosis induced directly by KCs. Results: miR-155 deficiency ameliorated liver IR injury, and inhibition of KCs by GdCl3 abolished this protective effect. miR-155 deficiency decreased CD80, CD86, and major histocompatibility complex class II expression in KCs after IR and tipped the M1/M2 balance toward an anti-inflammatory profile, where proinflammatory cytokine secretion was suppressed and IL-10 was enhanced. In addition, hepatocyte apoptosis was reduced in coculture with miR-155–deficient KCs in vitro. Conclusions: miR-155 deficiency plays an effective role in attenuating liver IR injury likely by regulating the activation and inflammatory response, as well as modifying the polarization of KCs. Abstract : The authors investigate the role of miR-155 on hepatic ischemic reperfusion injury (I/R) injury in a mouse model and further explore the underlying mechanism using primary macrophages. It is demonstrated that miR-155 deficiency protects liver from I/R injury by attenuating inflammatory response.