Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7. Issue 11 (November 2015)

• Record Type: Journal Article
• Authors: Lario, Luciana Daniela; Chaud, Luciana; Almeida, María das Graças; Converti, Attilio; Durães Sette, Lara; Pessoa, Adalberto
• Is Part Of: Fungal biology. Volume 119:Issue 11(2015:Nov.)
• Page Start: 1129
• Page End: 1136
• Publication Date: 2015-11
• Subjects: Cold-adapted yeast -- Enzyme characterization -- Extracellular protease; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2015.08.012
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular proteolytic activity in preliminary tests. R. mucilaginosa L7 was grown in Saboraud-dextrose medium at 25 °C, and the cell growth, pH of the medium, extracellular protease production and the glucose and protein consumption were determined as a function of time. The protease was then purified, and the effects of pH, temperature, and salt concentration on the catalytic activity and enzyme stability were determined. Enzyme production started at the beginning of the exponential phase of growth and reached a maximum after 48 h, which was accompanied by a decrease in the pH as well as reductions of the protein and glucose concentrations in the medium. The purified protease presented optimal catalytic activity at pH 5.0 and 50 °C. Finally, the enzyme was stable in the presence of high concentrations of NaCl. These characteristics are of interest for future studies and may lead to potential biotechnological applications that require enzyme activity and stability under acidic conditions and/or high salt concentrations. Highlights: We studied an acid protease secreted by a cold adapted yeast. The production of the protease was proportional to the yeast growth. The protease displayed a maximum activity around 45–55 °C. The enzyme retained more than 70 % of its maximal activity between 35 and 60 °C. The enzyme was stable in the presence of high concentrations of NaCl.