Effect of the three-dimensional organization of liver cells on the biogenesis of the γ-glutamyltransferase fraction pattern. (3rd July 2016)

• Record Type: Journal Article
• Authors: Corti, Alessandro; Fierabracci, Vanna; Caponi, Laura; Paolicchi, Aldo; Lorenzini, Evelina; Campani, Daniela; Belcastro, Eugenia; Franzini, Maria
• Is Part Of: Biomarkers. Volume 21:Number 5(2016)
• Page Start: 441
• Page End: 448
• Publication Date: 2016-07-03
• Subjects: Bile acids -- gel-filtration chromatography -- HepG2 cells -- liver parenchyma -- spheroids; Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2016.1153719
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Context Four gamma-glutamyltransferase (GGT) fractions with different molecular weights (big-, medium-, small- and free-GGT) are detectable in human plasma.Objective Verify if liver cells can release all four GGT fractions and if the spatial cell organization influences their release.Methods Hepatoma (HepG2) and melanoma (Me665/2/60) cells were cultured as monolayers or spheroids. GGT released in culture media was analysed by gel-filtration chromatography.Results HepG2 and Me665/2/60 monolayers released the b-GGT fraction, while significative levels of s-GGT and f-GGT were detectable only in media of HepG2-spheroids. Bile acids alone or in combination with papain promoted the conversion of b-GGT in s-GGT or f-GGT, respectively.Conclusions GGT is usually released as b-GGT, while s-GGT and f-GGT are likely to be produced in the liver extracellular environment by the combined action of bile acids and proteases.