Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus. Issue 6 (December 2015)

• Record Type: Journal Article
• Authors: Zhu, Yan Chun; Mitchell, Kara K.; Nazarian, Elizabeth J.; Escuyer, Vincent E.; Musser, Kimberlee A.
• Is Part Of: Molecular and cellular probes. Volume 29:Issue 6(2015)
• Page Start: 514
• Page End: 516
• Publication Date: 2015-12
• Subjects: Nontuberculous mycobacteria (NTM) -- Mycobacterium abscessus -- Real-time PCR -- Taqman -- Drug susceptibility -- Public health -- erm(41); Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2015.08.007
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: We have developed a single tube TaqMan ® real-time PCR assay that differentiates the full-length and truncated erm (41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus . A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance. Highlights: Developed a TaqMan ® real-time PCR assay to predict drug resistance in Mycobacterium abscessus . The assay is rapid, highly specific, sensitive, and meets NYS regulatory requirements. This assay predicts with 90.8% accuracy drug resistance to clarithromycin.