A cross-priming amplification assay coupled with vertical flow visualization for detection of Vibrio parahaemolyticus. Issue 6 (December 2015)

• Record Type: Journal Article
• Authors: Xu, Deshun; Wu, Xiaofang; Han, Jiankang; Chen, Liping; Ji, Lei; yan, Wei; Shen, Yuehua
• Is Part Of: Molecular and cellular probes. Volume 29:Issue 6(2015)
• Page Start: 527
• Page End: 530
• Publication Date: 2015-12
• Subjects: Vibrio parahaemolyticus -- Cross-priming amplification -- Vertical flow visualization -- Rapid detection -- Seafood; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2015.07.007
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. In this study, we developed a cross-priming amplification (CPA) assay coupled with vertical flow (VF) visualization for rapid and sensitive detection of V . parahaemolyticus . This assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). Small concentrations of V . parahaemolyticus (1.8 CFU/mL for pure cultures and 18 CFU/g for reconstituted samples) were detected within 1 h. CPA-VF can be applied at a large scale and can be used to detect V . parahaemolyticus strains rapidly in seafood and environmental samples, being especially useful in the field. Highlights: This detection method can be applied in field since the system only needs some simple equipments. The sealing device avoids cross-contamination between specimens, and the results are easy to interpret. This CPA-VF method is high sensitivity and specificity for the identification of V. parahaemolyticus in seafood samples.