Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol. Issue 12 (December 2015)

• Record Type: Journal Article
• Authors: Polburee, Pirapan; Yongmanitchai, Wichien; Lertwattanasakul, Noppon; Ohashi, Takao; Fujiyama, Kazuhito; Limtong, Savitree
• Is Part Of: Fungal biology. Volume 119:Issue 12(2015:Dec.)
• Page Start: 1194
• Page End: 1204
• Publication Date: 2015-12
• Subjects: Biodiversity -- Microbial lipid -- Rhodosporidium fluviale -- Waste glycerol; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2015.09.002
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: This study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. Two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. These strains were identified to be four ascomycetous yeast species i.e. Candida silvae, Kodamaea ohmeri, Meyerozyma caribbica, and Pichia manshurica, and five basidiomycetous yeast species i.e. Cryptococcus cf. podzolicus, Cryptococcus laurentii, Rhodosporidium fluviale, Rhodotorula taiwanensis, and Sporidiobolus ruineniae . Rhodosporidium fluviale DMKU-RK253 accumulated the highest quantity of lipid equal to 65.2 % of its biomass (3.9 g L −1 lipid and 6.0 g L −1 biomass) by shaking flask cultivation in crude glycerol. The main fatty acids in the accumulated lipid of this strain consisted of oleic acid, linoleic acid, and palmitic acid. Therefore, R. fluviale DMKU-RK253 has potential for producing lipid for biodiesel manufacturing using crude glycerol as a feedstock. Highlights: Rhodosporidium fluviale DMKU-RK253 accumulated high lipid (65 %) in crude glycerol. DMKU-RK253 has potential for producing lipid for biodiesel manufacturing. Four additional oleaginous yeast species were reported.