2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin Delays Palatal Shelf Elevation and Suppresses Wnt5a and Lymphoid Enhancing-Binding Factor 1 Signaling in Developing Palate. (January 2015) - Hu, Xiao
Objective: 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin contributes to cleft palate, but the cellular and molecular mechanisms responsible for the deleterious effect on the developing palate are unclear. Because Wnt signaling is associated with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in organ development, we wondered whether the malformation of the palate also results from altered Wnt signaling. Results: The 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin administration affected cell proliferation of the anteroposterior axis of the palatal shelf and delayed shelf elevation in mice. The activity of Wnt5a and lymphoid enhancing-binding factor 1 was inhibited by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in the developing palate. Conclusions: Downregulated Wnt5a and lymphoid enhancing-binding factor 1 are associated with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin–induced cleft palate. Moreover, delayed shelf elevation by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin is the crucial mechanism contributing to the high incidence of cleft palate. Our findings may help in elucidating the mechanisms of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin–induced cleft palate.