Interaction of hypericin with guanine-rich DNA: Preferential binding to parallel G-Quadruplexes. (September 2016)

• Record Type: Journal Article
• Authors: Zhang, Xin; Jin, Bing; Zheng, Wei; Zhang, Nan; Liu, Xiangjun; Bing, Tao; Wei, Yongbiao; Wang, Fuyi; Shangguan, Dihua
• Is Part Of: Dyes and pigments. Volume 132(2016)
• Page Start: 405
• Page End: 411
• Publication Date: 2016-09
• Subjects: Hypericin -- G-Quadruplexes -- DNA -- Binding model -- Molecular docking; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2016.05.009
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Hypericin (Hyp) is one of the principal active constituents of a traditional folk medicine, Hypericum (Saint John's wort), and possesses variety biological activities. The interaction of Hyp with DNA may play an important role in biological activity, however only a few investigations were reported on this issue. In this paper, we studied the interaction of Hyp with different DNA forms. Hyp was found to selectively bind parallel DNA G-Quadruplexes (G4) over other DNA forms, such as ss-/ds-DNA, antiparallel G4s and mix-type G4s. Binding studies suggest that Hyp bind on G-quartet surface of G4s through end-stacking interaction. These results provide new information for the investigation of action mechanism of Hyp. Graphical abstract: Highlights: Hypericin was first reported to specifically bind to parallel G-Quadruplexes. Hypericin binds to parallel G-Quadruplexes through end-stacking mode. Hypericin binding to parallel G-Quadruplexes causes great fluorescence enhancement.