Epithelial–mesenchymal transition-related genes are linked to aggressive local recurrence of hepatocellular carcinoma after radiofrequency ablation. Issue 1 (28th May 2016)

• Record Type: Journal Article
• Authors: Iwahashi, Shuichi; Shimada, Mitsuo; Utsunomiya, Tohru; Imura, Satoru; Morine, Yuji; Ikemoto, Tetsuya; Takasu, Chie; Saito, Yu; Yamada, Shinichiro
• Is Part Of: Cancer letters. Volume 375:Issue 1(2016)
• Page Start: 47
• Page End: 50
• Publication Date: 2016-05-28
• Subjects: Hepatocellular carcinoma (HCC) -- Radiofrequency ablation (RFA) -- Epithelial–Mesenchymal Transition (EMT) -- MicroRNA; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.02.041
• Languages: English
• ISSNs: 0304-3835

Abstract

Highlights: Aggressive progression in residual liver tumors after RFA was studied. We focused on epithelial–mesenchymal transition (EMT)-related genes and miRNAs. Increased expression of EMT-related genes was through down-regulation of miRNAs. Abstract: We reported that poor prognoses of hepatocellular carcinoma (HCC) patients after radiofrequency ablation (RFA) are owing to up-regulation of expression of hypoxia-inducible factor-1 and epithelial cell adhesion molecule. We investigated aggressive progression in residual liver tumors (RLTs) after RFA to focus on expression of epithelial–mesenchymal transition (EMT)-related genes and miRNAs. Ten patients with recurrent HCC post-RFA who underwent hepatectomy (RFA group) and 78 patients with HCC without prior RFA (non-RFA group) were enrolled. We examined expression of transforming growth factor (TGF)-β, Twist, vimentin, and Snail-1 mRNAs in tumor tissues, and expression of miR-34a and miR-200c. Expression of TGF-β, Twist and Snail-1 in the RFA group was significantly higher than that in the non-RFA group (P < 0.05); vimentin expression in the RFA group was higher than that in the non-RFA group (P = 0.07). Expression of miR-200c and miR-34a in the RFA group was significantly lower than that in the non-RFA group (miR-200c: P = 0.04; miR-34a: P < 0.01). Increased expression of EMT markers through down-regulation of miRNA expression in RLTs after RFA may be related to poor prognoses of HCC patients with aggressive local recurrence after RFA.