Immune activation effects of Eurotium cristatum on T cells through NF-κB signaling pathways in humans. Issue 3 (4th May 2017)

• Record Type: Journal Article
• Authors: Yan, Zheng-Fei; Lin, Pei; Kook, MooChang; Yi, Tae-Hoo; Li, Chang-Tian
• Is Part Of: Food and agricultural immunology. Volume 28:Issue 3(2017)
• Page Start: 388
• Page End: 402
• Publication Date: 2017-05-04
• Subjects: Fuzhuan brick-tea -- immunotherapeutic agent -- NF-κB signaling pathway -- nuclear translocation -- T cell; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2017.1293012
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: The immunization effect of Eurotium cristatum isolated from Fuzhuan brick-tea was evaluated. Ethyl acetate extraction (E2) had an increasing effect on T cell subset proportions. And then four compounds (physcion, echinulin, catenarin and emodin) were isolated from E. cristatum . The immunization effect of echinulin was observed through determining T cell subsets' percentage, DNA synthesis and cytokine levels. It suggested that the immunization effect of compounds might work in relation to its structure. In addition, echinulin accelerated protein of p65, IκBα upregulation and nuclear translocation of p65 by the NF-κB signaling pathway. The result showed that the immunization effect of echinulin is closely related to the activation of NF-κB activity. Meanwhile echinulin increased transcript levels of p65 and IκBα in T cells at 200 μM (middle dosages group). It demonstrated that the immunization effection of echinulin on T cells is enhanced by activating NF-κB signaling pathway.