Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. (January 2016)

• Record Type: Journal Article
• Authors: Michelin, Michele; Ximenes, Eduardo; de Lourdes Teixeira de Moraes Polizeli, Maria; Ladisch, Michael R.
• Is Part Of: Bioresource technology. Volume 199(2016)
• Page Start: 275
• Page End: 278
• Publication Date: 2016-01
• Subjects: Inhibition -- Deactivation -- Enzyme -- Phenolic compounds -- Sugarcane bagasse; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.08.120
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Liquid hot water pretreatment of bagasse releases phenolic compounds. Phenolics in aqueous filtrates inhibit/deactivate cellulases and hemicellulases. Aqueous filtrates deactivate β-glucosidase and β-xylosidase, but less strongly. Phenolics from acetone extracts strongly deactivate β-glucosidase and β-xylosidase. Abstract: This work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200 °C. The dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/L as temperature increased from 180 to 200 °C. The suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the β-glucosidase or β-xylosidase components of cellulase and hemicellulase enzyme by acetone extract at 0.2–0.65 mg phenolics/mg enzyme protein and deactivation of cellulases and hemicellulases by the water soluble components in vacuum filtrate at 0.05–2 mg/mg. Inhibition was a function of the type of enzyme and the manner in which the phenolics were extracted from the bagasse.