Leptin regulates energy metabolism in MCF-7 breast cancer cells. (March 2016)

• Record Type: Journal Article
• Authors: Blanquer-Rosselló, Mª del Mar; Oliver, Jordi; Sastre-Serra, Jorge; Valle, Adamo; Roca, Pilar
• Is Part Of: International journal of biochemistry & cell biology. Volume 72(2016:Mar.)
• Page Start: 18
• Page End: 26
• Publication Date: 2016-03
• Subjects: Breast cancer -- Leptin -- Warburg effect -- Metabolism -- Lipid oxidation -- Glycolysis; 2-DG 2-deoxyglucose -- AMPK 5′ AMP-activated protein kinase -- FAT/CD36 fatty acid translocase or cluster of differentiation 36 -- CPT1 carnitine palmitoyltransferase I -- CS citrate synthase -- G6PDH glucose-6-phosphate dehydrogenase -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- GLUT2 glucose transporter 2 -- LDH lactate dehydrogenase -- PDH pyruvate dehydrogenase -- pAMPK phosphorylated 5′ AMP-activated protein kinase -- OCR oxygen consumption rate -- OXPHOS oxidative phosphorylation -- PC pyruvate carboxylase -- PDH pyruvate dehydrogenase -- PPARα peroxisome proliferator-activated receptor alpha; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2016.01.002
• Languages: English
• ISSNs: 1357-2725

Abstract

Abstract: Obesity is known to be a poorer prognosis factor for breast cancer in postmenopausal women. Among the diverse endocrine factors associated to obesity, leptin has received special attention since it promotes breast cancer cell growth and invasiveness, processes which force cells to adapt their metabolism to satisfy the increased demands of energy and biosynthetic intermediates. Taking this into account, our aim was to explore the effects of leptin in the metabolism of MCF-7 breast cancer cells. Polarographic analysis revealed that leptin increased oxygen consumption rate and cellular ATP levels were more dependent on mitochondrial oxidative metabolism in leptin-treated cells compared to the more glycolytic control cells. Experiments with selective inhibitors of glycolysis (2-DG), fatty acid oxidation (etomoxir) or aminoacid deprivation showed that ATP levels were more reliant on fatty acid oxidation. In agreement, levels of key proteins involved in lipid catabolism (FAT/CD36, CPT1, PPARα) and phosphorylation of the energy sensor AMPK were increased by leptin. Regarding glucose, cellular uptake was not affected by leptin, but lactate release was deeply repressed. Analysis of pyruvate dehydrogenase (PDH), lactate dehydrogenase (LDH) and pyruvate carboxylase (PC) together with the pentose-phosphate pathway enzyme glucose-6 phoshate dehydrogenase (G6PDH) revealed that leptin favors the use of glucose for biosynthesis. These results point towards a role of leptin in metabolic reprogramming, consisting of an enhanced use of glucose for biosynthesis and lipids for energy production. This metabolic adaptations induced by leptin may provide benefits for MCF-7 growth and give support to the reverse Warburg effect described in breast cancer.