Fish chemokines 14, 20 and 25: A comparative statement on computational analysis and mRNA regulation upon pathogenic infection. Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Arockiaraj, Jesu; Bhatt, Prasanth; Kumaresan, Venkatesh; Dhayanithi, Nagarajan Balachandran; Arshad, Aziz; Harikrishnan, Ramasamy; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah
• Is Part Of: Fish & shellfish immunology. Volume 47:Issue 1(2015:Nov.)
• Page Start: 221
• Page End: 230
• Publication Date: 2015-11
• Subjects: Murrel -- Chemokine -- Gene expression -- Fungus -- Bacteria; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.09.015
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In this study, we reported a molecular characterization of three CC chemokines namely, Cs CC-Chem14, Cs CC-Chem20 and Cs CC-Chem25 which are were identified from the established cDNA library of striped murrel Channa striatus. Multiple sequence alignment of all the three chemokines revealed the presence of gene specific domains and motifs including small cytokine domain, IL8 like domain, receptor binding site and glycosaminoglycan (GAG) binding sites. Three dimensional structures of the chemokines under study showed an important facet on their anti-microbial property. Tissue specific mRNA expression showed that the Cs CC-Chem14 is highly expressed in spleen, Cs CC-Chem20 in liver and Cs CC-Chem25 in trunk kidney. On challenge C. striatus with oomycete fungus Aphanomyces invadans, both Cs CC-Chem20 and Cs CC-Chem25 showed significant (P < 0.05) up-regulation compared to Cs CC-Chem14. The increase in the expression levels of Cs CC-Chem20 and Cs CC-Chem25 due to infection showed that they are antimicrobial proteins. But considering the Cs CC-Chem14 expression, it is found to be a constitutive chemokine and is involved in homeostatic function in spleen of C. striatus . C. striatus challenged with bacteria Aeromonas hydrophila also exhibited different up-regulation pattern in all the three chemokines at various time points. However, extensive studies are required to determine the functional activities of Cs CC-Chem14, Cs CC-Chem20 and Cs CC-Chem25 in vitro and in vivo to gain more knowledge at the molecular and proteomic levels. Highlights: A comparative account on Channa striatus CC chemokine 14, 20 and 25 was addressed. Cs CC-Chem20 and Cs CC-Chem25 are antimicrobial agents. Cs CC-Chem14 was constitutive chemokine involved in homeostasis. All three Chemokines regulated upon microbial infection.