Allograft inflammatory factor-1 in grass carp (Ctenopharynogodon idella): Expression and response to cadmium exposure. Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Zhao, Yanying; Li, Ruiwen; Lin, Yaqiu
• Is Part Of: Fish & shellfish immunology. Volume 47:Issue 1(2015:Nov.)
• Page Start: 444
• Page End: 449
• Publication Date: 2015-11
• Subjects: Allograft inflammatory factor-1 -- AIF-1 -- Grass carp -- Cadmium; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.08.034
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Allograft Inflammatory Factor-1 (AIF-1) is an inflammation responsive protein that is mainly produced by immunocytes. As a pro-inflammatory cytokine, AIF-1 is a key moderator in host immune defense reaction. However, the inflammatory properties of AIF-1 in freshwater fish still hasn't been clearly elucidated. In the present study, AIF-1 was identified from grass carp ( Ctenopharynogodon idella ). It's transcript was found in all examined tissues including brain, spleen, kidney, liver, heart, while a relative low level in red muscle, gill, thymus, white muscle, intestine and fin. Furthermore, AIF-1 transcription and expression level decreased in spleen and didn't change a lot in kidney with cadmium induction, respectively. The result indicated that grass carp AIF-1 might be involved in cadmium-induced stress. Highlights: Allograft inflammatory factor-1 (AIF-1) gene was cloned from grass carp. AIF-1 transcript was found in all examined tissues. AIF-1 protein was purified and polyclonal antibody against it was produced. Cd decreased level of AIF-1 mRNA in spleen, and didn't changed it a lot in kidney. Cd decreased level of AIF-1 protein in spleen, and didn't changed it a lot in kidney.