Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7 cells. (August 2016)

• Record Type: Journal Article
• Authors: Lutter, Anne-Helen; Hempel, Ute; Anderer, Ursula; Dieter, Peter
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 111(2016:Aug.)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-08
• Subjects: αMEM; Alpha Minimum Essential Medium -- cAMP cyclic adenosine monophosphate -- DMEM Dulbecco's Modified Eagle's Medium -- EDTA ethylenediaminetetraacetic acid -- ERK extracellular signal-regulated kinases -- FBS fetal bovine serum -- GPCR G-protein-coupled receptor -- hPBMC human peripheral blood mononuclear cell -- MCSF macrophage colony-stimulating factor -- OC osteoclast -- ODEM osteoblast-derived extracellular matrix -- PBS phosphate buffered saline -- PGE2 prostaglandin E2 -- PI3K posphatidylinositol-3-kinase -- PKA protein kinase A -- PKC protein kinase C -- PMSF phenylmethanesulfonyl fluoride -- NaOH sodium hydroxide -- RANK receptor activator of nuclear factor κB -- RANKL receptor activator of nuclear factor κB ligand -- TRAP tartrate-resistant acid phosphatase -- Tris–HCl Tris(hydroxymethyl)aminomethane hydrochloride; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2016.03.017
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: Osteoclasts are large bone-resorbing cells of hematopoietic origin. Their main function is to dissolve the inorganic component hydroxyapatite and to degrade the organic bone matrix. Prostaglandin E2 (PGE2 ) indirectly affects osteoclasts by stimulating osteoblasts to release factors that influence osteoclast activity. The direct effect of PGE2 on osteoclasts is still controversial. To study the influence of PGE2 on osteoclast activity, human peripheral blood monocytes (hPBMC) and mouse RAW264.7 cells were cultured on osteoblast-derived extracellular matrix. hPBMC and RAW264.7 cells were differentiated by the addition of macrophage colony-stimulation factor and receptor activator of NFκB ligand and treated with PGE2 before and after differentiation induction. The pit area, an indicator of resorption activity, and the activity of tartrate-resistant acid phosphatase were dose-dependently inhibited when PGE2 was present ab initio, whereas the resorption activity remained unchanged when the cells were exposed to PGE2 from day 4 of culture. These results lead to the conclusion that PGE2 treatment inhibits only the differentiation of precursor osteoclasts whereas differentiated osteoclasts are not affected. Highlights: PGE2 is an important pro-inflammatory mediator regulating osteoclast function. The effect of PGE2 on TRAP and resorptive activity of osteoclastic cells was studied. PGE2 inhibited the differentiation of human and mouse osteoclast precursor cells. PGE2 had no influence on already differentiated human and mouse osteoclasts. Downregulation of PGE2 receptors enables osteoclasts function during inflammation.