Consumption of protein-enriched milk has minor effects on inflammation in older adults—A 12-week double-blind randomized controlled trial. (March 2017)

• Record Type: Journal Article
• Authors: Gjevestad, Gyrd O.; Ottestad, Inger; Biong, Anne Sofie; Iversen, Per Ole; Retterstøl, Kjetil; Raastad, Truls; Skålhegg, Bjørn S.; Ulven, Stine M.; Holven, Kirsten B.
• Is Part Of: Mechanisms of ageing and development. Volume 162(2017)
• Page Start: 1
• Page End: 8
• Publication Date: 2017-03
• Subjects: B2M beta-2-microglobulin -- BMI body mass index -- CCL chemokine (C-C motif) ligand -- cDNA complimentary deoxyribonucleic acid -- CXCL16 chemokine (C-X-C motif) ligand 16 -- DPP4 dipeptidyl-peptidase 4 -- DXA dual energy X-ray absorptiometry -- g gravity -- Gadd45 growth arrest and DNA-damage-inducible, alpha -- hs-CRP high sensitive C-reactive protein -- IFNG interferon gamma -- IGF1 insulin-like growth factor 1 -- IL interleukin -- IL1RN/IL1Ra interleukin 1 receptor antagonist -- liver X receptor alpha LXRα -- mRNA messenger RNA -- NF-kB nuclear factor kappa-light-chain-enhancer of activated B cells -- NR1H3 nuclear receptor subfamily, group H, member 3 -- PBMC peripheral blood mononuclear cells -- PDK4 pyruvate dehydrogenase kinase, isozyme 4 -- qPCR quantitative polymerase chain reaction -- rpm rounds per minute -- Runx2 runt-related transcription factor 2 -- sTNFRSF1A soluble tumor necrosis factor receptor 1 -- TBP TATA box binding protein -- TLDA TaqMan Low-Density array -- TLR toll-like receptor -- TNFα tumor necrosis factor alpha -- TNFSF11 tumor necrosis factor ligand superfamily member 11 -- TNFRSF1A tumor necrosis factor receptor superfamily, member 1A -- TNFRSF11A tumor necrosis factor receptor superfamily, member 11A; Milk protein -- Older adults -- High-protein -- Inflammation -- Peripheral blood mononuclear cells -- mRNA; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2017.01.011
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Compared with carbohydrates, 12 weeks of consuming low-fat, protein-enriched milk had minor effects on inflammatory markers. The mRNA Expression levels of NR1H3 and INFG differed between the study groups. The mRNA expression of TNFRSF1A was reduced, while the expression of DPP4 was increased in the control group. There were no differences in the mRNA expression of TNFRSF1A and DPP4 between the groups. The serum level of sTNFRSF1A increased in both groups, while the level of TNFα increased in the control group only. Abstract: Introduction: Aging is associated with increased levels of circulating inflammatory markers and reduced muscle mass and strength. Objective: We investigated whether intake of protein-enriched milk for 12 weeks would influence markers of inflammation among adults ≥70 years of age with reduced physical strength. Methods: In a double-blind randomized controlled intervention study, subjects were randomly allocated into two groups, receiving a protein-enriched milk (2 × 20 g protein/d, n = 14, mean (±SD) age 76.9 ± 4.9 yrs) or an isocaloric carbohydrate drink (n = 17, age 77.7 ± 4.8 yrs) for 12 weeks. We measured serum and mRNA expression levels of inflammatory markers in PBMCs. Results: Significant differences in the mRNA expression of nuclear receptor subfamily, group H, member 3 ( NR1H3, encoding the LXRα transcription factor) and interferon gamma ( INFG) were observed between groups. The mRNA level of TNFRSF1A was significantly reduced, while the mRNA level of dipeptidyl-peptidase 4 ( DPP4) was significantly increased, in the control group. The serum level of TNFα increased significantly in the control group, while sTNFRSF1A increased significantly in both groups, but with no significant differences between groups. Conclusion: Consumption of a low-fat, protein-enriched milk for 12 weeks had minor effects on inflammatory related markers in older adults compared to an isocaloric carbohydrate drink.