Isolation, purification, characterization and immunostimulatory activity of polysaccharides derived from American ginseng. (20th January 2017)

• Record Type: Journal Article
• Authors: Yu, Xiao-Hong; Liu, Ying; Wu, Xian-Ling; Liu, Li-Zhai; Fu, Wei; Song, Dan-Dan
• Is Part Of: Carbohydrate polymers. Volume 156(2017)
• Page Start: 9
• Page End: 18
• Publication Date: 2017-01-20
• Subjects: American ginseng -- Polysaccharide -- Purification -- Characterization -- Immunostimulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.08.092
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Efficient methods for deproteinizing and decolorizing polysaccharides demonstrated. Polyamide chromatography is the most efficient deproteinization method. Resin S-8 is the most efficient decolorization method. Salt-eluted polysaccharide has more significant immunostimulatory effects. Polysaccharide fraction SPS-3 showed the most immunostimulatory activity. Abstract: In this study, crude American ginseng polysaccharide (AGPS) was extracted with hot water and preliminarily purified by using resin S-8 and Polyamide columns. Then, it was further purified and separated by DEAE-Sepharose CL-6B and Sepharose CL-6B chromatography, respectively. Five main fractions were obtained, named WPS-1, WPS-2, SPS-1, SPS-2 and SPS-3. Their homogeneities and structural characteristics were elucidated based on UV–vis spectroscopy, High Performance Gel Filtration Chromatography (HPGFC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectrum (IR), and NMR Spectroscopy methods. Furthermore, the immunostimulatory effects of these fractions upon splenic lymphocyte proliferation, macrophage phagocytosis and nitric oxide (NO) production, were investigated in vitro. The results indicated that their stimulations could be ordered as SPS-3 > SPS-1 > CPS (crude polysaccharides) > WPS-1 > WPS-2 > SPS-2. Among them, SPS-3 showed more potent immunomodulatory activity and could be explored as a potential immunopotentiating agent for use in functional food or medicine.