Immobilization of purified β-glucuronidase on ZnO nanoparticles for efficient biotransformation of glycyrrhizin in ionic liquid/buffer biphasic system. (27th April 2017)

• Record Type: Journal Article
• Authors: Kaleem, Imdad; Rasool, Aamir; Lv, Bo; Riaz, Naveeda; Hassan, Jalees Ul; Manzoor, Robina; Li, Chun
• Is Part Of: Chemical engineering science. Volume 162(2017)
• Page Start: 332
• Page End: 340
• Publication Date: 2017-04-27
• Subjects: β-glucuronidase -- ZnO nanoparticles -- Immobilization -- Ionic liquid -- Operational stability; Chemical engineering -- Periodicals; Génie chimique -- Périodiques; Chemical engineering; Periodicals; Electronic journals; 660
• DOI: 10.1016/j.ces.2016.12.074
• Languages: English
• ISSNs: 0009-2509

Abstract

Highlights: Purified recombinant β-glucuronidase (PGUS-E) was immobilized on ZnO-NP. The immobilization efficiency was compared in buffer and ionic liquids. PGUS-E displayed higher stability in IL/buffer biphasic system than buffer. Recovery rate of IL medium ([Bmim]PF6) was higher than the other IL media. Abstract: Purified recombinant β-glucuronidase (PGUS-E) from Aspergillus oryzae Li-3 was immobilized on the zinc oxide nanoparticles (ZnO-NP) for glycyrrhizin (GL) biotransformation. The optimal loading efficiency of the PGUS-E on ZnO-NP was 6.52 U/mg with an average of 85.83% immobilization yield. The adsorption of the PGUS-E on ZnO-NP was confirmed using scanning electron microscope (SEM) and fourier transform infrared (FTIR) spectroscope. The comparative catalytic efficiency of the immobilized PGUS-E was evaluated in the ionic liquids (ILs) media and buffer. The higher catalytic efficiency of the immobilized PGUS-E was recorded in the hydrophobic ionic liquid [Bmim]PF6 (20% volumetric ratio) compared with other ILs media and pure buffer. The temperature and pH profiles of the immobilized PGUS-E were also determined for ionic liquid (ILs) media and pure buffer. The higher operational stability of the immobilized PGUS-E was observed in the IL co-solvent medium than the pure buffer medium; and after 8 repeated uses an average 30.54% and 7.42% of its catalytic activity was respectively retained. The recovery rate of the IL medium ([Bmim]PF6 ) was as high as 76.11%. The measurement of the enzyme kinetic parameters and activation energy also explicitly display the superiority of the IL co-solvent media over the monophasic media.