Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar. (September 2016)

• Record Type: Journal Article
• Authors: Aung, Htin Lin; Tun, Thanda; Moradigaravand, Danesh; Köser, Claudio U.; Nyunt, Wint Wint; Aung, Si Thu; Lwin, Thandar; Thinn, Kyi Kyi; Crump, John A.; Parkhill, Julian; Peacock, Sharon J.; Cook, Gregory M.; Hill, Philip C.
• Is Part Of: Journal of global antimicrobial resistance. Volume 6(2016:Sep.)
• Page Start: 113
• Page End: 117
• Publication Date: 2016-09
• Subjects: Whole-genome sequencing -- Drug-resistant TB -- Myanmar; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2016.04.008
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Drug-resistant tuberculosis (TB) is a major health threat in Myanmar. The first whole-genome sequencing study of drug-resistant TB from Myanmar. Introduction of second-line drug susceptibility testing as part of routine diagnosis in Myanmar is needed. Abstract: Drug-resistant tuberculosis (TB) is a major health threat in Myanmar. An initial study was conducted to explore the potential utility of whole-genome sequencing (WGS) for the diagnosis and management of drug-resistant TB in Myanmar. Fourteen multidrug-resistant Mycobacterium tuberculosis isolates were sequenced. Known resistance genes for a total of nine antibiotics commonly used in the treatment of drug-susceptible and multidrug-resistant TB (MDR-TB) in Myanmar were interrogated through WGS. All 14 isolates were MDR-TB, consistent with the results of phenotypic drug susceptibility testing (DST), and the Beijing lineage predominated. Based on the results of WGS, 9 of the 14 isolates were potentially resistant to at least one of the drugs used in the standard MDR-TB regimen but for which phenotypic DST is not conducted in Myanmar. This study highlights a need for the introduction of second-line DST as part of routine TB diagnosis in Myanmar as well as new classes of TB drugs to construct effective regimens.