Interleukin-1β induces sleep independent of prostaglandin D2 in rats and mice. (6th January 2017)

• Record Type: Journal Article
• Authors: Zhang, Bin-Jia; Shao, Shu-Rong; Aritake, Kosuke; Takeuchi, Atsuko; Urade, Yoshihiro; Huang, Zhi-Li; Lazarus, Michael; Qu, Wei-Min
• Is Part Of: Neuroscience. Volume 340(2017)
• Page Start: 258
• Page End: 267
• Publication Date: 2017-01-06
• Subjects: COX-2 cyclooxygenase-2 -- CSF cerebrospinal fluid -- DMSO dimethyl sulfoxide -- DP1R DP1 receptors -- EEG electroencephalogram -- EMG electromyogram -- IL-1β Interleukin 1 beta -- KO knockout -- LC–MS/MS liquid chromatography–tandem mass spectrometry -- L-PGDS lipocalin-type PGD synthase -- LPS lipopolysaccharide -- NREM non-rapid eye movement -- PG prostaglandins -- PGEM PGE2 metabolite 9, 15-dioxo-11α-hydroxy-2, 3, 4, 5-tetranorprostan-1, 20-dioic acid -- PGDM PGD2 metabolite 11, 15-dioxo-9α-hydroxy-2, 3, 4, 5-tetranorprostan-1, 20-dioic acid -- SWA slow-wave activity -- TMN tuberomammamillary nucleus -- VLPO ventrolateral preoptic area -- WT wild-type; cytokine -- prostaglandin D2 -- sleep -- body temperature -- slow-wave activity -- COX-2 inhibition; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.09.053
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: We studied whether IL-1β-induced sleep is mediated by prostaglandins. IL-1β induces sleep in rats and mice with or without COX-2 inhibitor treatment. IL-1β induces sleep in PGD2 receptor subtype DP1 KO mice. IL-1β induced sleep is independent of PGD2 and other COX-2-derived prostaglandins. Abstract: Interleukin-1β (IL-1β) and prostaglandin (PG) D2 are endogenous sleep-promoting substances. Since it was reported that a highly selective cyclooxygenase-2 (COX-2) inhibitor, NS398, completely inhibited IL-1β-induced sleep in rats, IL-1β-induced sleep had been believed to be mediated by prostanoids, most probably PGD2 . However, in the present study, pretreatment of rats with NS398 (3 mg/kg) did not suppress the 64.2% increased non-rapid eye movement (non-REM, NREM) sleep during infusion of IL-1β (10 ng) for 6 h in the nocturnal (active) period between 23:00 and 5:00 into the subarachnoid space of the PGD2 -sensitive sleep-promoting zone of the basal forebrain. Meanwhile, IL-1β at doses of 1.7 and 5 μg/kg also significantly increased NREM sleep for 6 h after intraperitoneal injection at 20:00 (light-off time) by 76.8% and 121.1%, respectively, in wild-type (WT) mice, by 67.7% and 147.3%, respectively, in WT mice pretreated with NS398 (5 mg/kg) and by 108.9% and 121.6%, respectively, in PGD2 receptor (DP1 R) knockout mice. These results indicate that IL-1β-induced NREM sleep is independent of the PGD2 /DP1 R system and other COX-2-derived prostaglandins in rats and mice.