Breathable tissue engineering scaffolds: An efficient design-optimization by additive manufacturing. (2018) - Touri, Maria
Abstract: Supplying sufficient oxygen to engineered tissue, has been a major challenge for overcoming the effect of hypoxia. Using of oxygen producing scaffolds has opened a window to prevent ischemia-related cell necrosis. In this study, biphasic calcium phosphate (BCP) porous structures consisting of a 3-D tetragonal mesh of interpenetrating ceramic rods were fabricated by robocasting technique. Calcium peroxide (CPO, 3 wt%), was encapsulated within the polycaprolactone (PCL) coating layer through a dip-coating method. The presence of CPO particles in coated scaffolds played the role of a driving force for formation of apatite in SBF test then compared to uncoated scaffold, the coated scaffold presented a better ability of precipitation. The sustained oxygen release ensured the effectiveness of the coating-scaffold design as an oxygen delivery system. The coated scaffolds with 3% CPO, could also improve osteoblasts viability and proliferation. These findings show that the prepared scaffolds can be a great candidate in bone tissue engineering with promoting of bone ingrowth.