Seasonal variation in thermal tolerance, oxygen consumption, antioxidative enzymes and non-specific immune indices of Indian hill trout, Barilius bendelisis (Hamilton, 1807) from central Himalaya, India. (August 2015)

• Record Type: Journal Article
• Authors: Sharma, Neeraj Kumar; Akhtar, M.S.; Pandey, Nityanand; Singh, Ravindra; Singh, Atul Kumar
• Is Part Of: Journal of thermal biology. Volume 52(2015)
• Page Start: 166
• Page End: 176
• Publication Date: 2015-08
• Subjects: Season -- Thermal tolerance -- Oxygen consumption -- Respiratory burst activity -- WBC -- Neutrophil -- Monocyte -- Antioxidative enzymes -- Barilius bendelisis; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2015.07.005
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: We studied the season dependent thermal tolerance, oxygen consumption, respiratory burst response and antioxidative enzyme activities in juveniles of Barilius bendelisis . The critical thermal maximum (CTmax), lethal thermal maximum (LTmax), critical thermal minimum (CTmin) and lethal thermal minimum (LTmin) were significantly different at five different seasons viz. winter (10.64 °C), spring (16.25 °C), summer (22.11 °C), rainy (20.87 °C) and autumn (17.77 °C). The highest CTmax was registered in summer (36.02 °C), and lowest CTmin was recorded during winter (2.77 °C). Water temperature, dissolved oxygen and pH were strongly related to CTmax, LTmax, CTmin and LTmin suggesting seasonal acclimatization of B. bendelisis . The thermal tolerance polygon area of the B. bendelisis juveniles within the range of seasonal temperature (10.64–22.11 °C) was calculated as 470.92 °C 2 . Oxygen consumption rate was significantly different ( p <0.05) between seasons with maximum value during summer (57.66 mg O2 /kg/h) and lowest in winter (32.60 mg O2 /kg/h). Total white blood cell count including neutrophil and monocytes also showed significant difference ( p <0.05) between seasons with maximum value during summer and minimum number in winter and were found correlated to temperature, dissolved oxygen, pH and respiratory burst activity. Respiratory burst activity of blood phagocytes significantly differed ( p <0.05) among seasons with higher value during summer (0.163 OD540 nm ) and minimum in winter season (0.054 OD540 nm ). The activity of superoxide dismutase, catalase and glutathione-s-transferase both in liver and gill, also varied significantly ( p <0.05) during different seasons. Overall results of this study suggest that multiple environmental factors play a role in seasonal acclimation in B. bendelisis, which modulate the thermal tolerance, oxygen consumption, respiratory burst activity and status of anti-oxidative potential in wild environment. Highlights: We studied thermal tolerance and physio-immunological indices in Barilius bendelisis . Highest CTmax and LTmax were observed in summer season. Oxygen consumption rate was maximum during summer and minimum in winter season. Higher leukocyte and differential count were observed during summer and rainy seasons. Upsurge in NBT activity in summer was due to increase in neutrophils and monocytes. Activities of SOD, catalase and GST were altered by seasonal environmental factors.