Single molecule approaches for studying gene regulation in metabolic tissues. (19th September 2018)

• Record Type: Journal Article
• Authors: Farack, Lydia; Egozi, Adi; Itzkovitz, Shalev
• Is Part Of: Diabetes, obesity & metabolism. Volume 20(2018)Supplement 2
• Page Start: 145
• Page End: 156
• Publication Date: 2018-09-19
• Subjects: bursty transcription -- gene expression noise -- mRNA degradation -- single molecule fluorescence in situ hybridization -- transcription rate; Diabetes -- Periodicals; Obesity -- Periodicals; Metabolism -- Disorders -- Periodicals; Clinical pharmacology -- Periodicals; 616.462
• DOI: 10.1111/dom.13390
• Languages: English
• ISSNs: 1462-8902

Abstract

Abstract : Gene expression in metabolic tissues can be regulated at multiple levels, ranging from the control of promoter accessibilities, transcription rates, mRNA degradation rates and mRNA localization. Modulating these processes can differentially affect important performance criteria of cells. These include precision, cellular economy, rapid response and maintenance of DNA integrity. In this review we will describe how distinct strategies of gene regulation impact the trade‐offs between the cells' performance criteria. We will highlight tools based on single molecule visualization of transcripts that can be used to measure promoter states, transcription rates and mRNA degradation rates in intact tissues. These approaches revealed surprising recurrent patterns in mammalian tissues, that include transcriptional bursting, nuclear retention of mRNA, and coordination of mRNA lifetimes to facilitate rapid adaptation to changing metabolic inputs. The ability to characterize gene expression at the single molecule level can uncover the design principles of gene regulation in metabolic tissues such as the liver and the pancreas.