Stunting correlates with high salivary and serum antibody levels after 13-valent pneumococcal conjugate vaccination of Venezuelan Amerindian children. Issue 20 (29th April 2016)

• Record Type: Journal Article
• Authors: Verhagen, Lilly M.; Hermsen, Meyke; Rivera-Olivero, Ismar; Sisco, María Carolina; Pinelli, Elena; Hermans, Peter W.M.; Berbers, Guy A.M.; Waard, Jacobus H. de; Jonge, Marien I. de
• Is Part Of: Vaccine. Volume 34:Issue 20(2016)
• Page Start: 2312
• Page End: 2320
• Publication Date: 2016-04-29
• Subjects: 13-Valent pneumococcal conjugate vaccination -- Indigenous children -- Chronic malnutrition -- Saliva -- Venezuela; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2016.03.066
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: In Venezuelan children, PCV13 salivary and serum antibody levels correlated well. Saliva may be used as a read-out for PCV13 vaccine response. Stunted children had higher specific serum and salivary PCV13 antibody levels. More studies addressing the effect of malnutrition on vaccine induced responses are needed. Abstract: Objective: To determine the impact of pre-vaccination nutritional status on vaccine responses in Venezuelan Warao Amerindian children vaccinated with the 13-valent pneumococcal conjugate vaccine (PCV13) and to investigate whether saliva can be used as read-out for these vaccine responses. Methods: A cross-sectional cohort of 504 Venezuelan Warao children aged 6 weeks – 59 months residing in nine geographically isolated Warao communities were vaccinated with a primary series of PCV13 according to Centers for Disease Control and Prevention (CDC)-recommended age-related schedules. Post-vaccination antibody concentrations in serum and saliva of 411 children were measured by multiplex immunoassay. The influence of malnutrition present upon vaccination on post-vaccination antibody levels was assessed by univariate and multivariable generalized estimating equations linear regression analysis. Results: In both stunted (38%) and non-stunted (62%) children, salivary antibody concentrations correlated well with serum levels for all serotypes with coefficients varying from 0.61 for serotype 3–0.80 for serotypes 5, 6A and 23F (all p < 0.01). Surprisingly, higher serum and salivary antibody levels were observed with increasing levels of stunting in children for all serotypes. This was statistically significant for 5/13 and 11/13 serotype-specific serum and saliva IgG concentrations respectively. Conclusion: Stunted Amerindian children showed generally higher antibody concentrations than well-nourished children following PCV13 vaccination, indicating that chronic malnutrition influences vaccine response. Saliva samples might be useful to monitor serotype-specific antibody levels induced by PCV vaccination. This would greatly facilitate studies of vaccine efficacy in rural settings, since participant resistance generally hampers blood drawing. The study was registered in a primary registry of the World Health Organization with identifier number RPCEC00000158.