Regulation of cuticle formation during fruit development and ripening in 'Newhall' navel orange (Citrus sinensis Osbeck) revealed by transcriptomic and metabolomic profiling. (February 2016)

• Record Type: Journal Article
• Authors: Wang, Jinqiu; Sun, Li; Xie, Li; He, Yizhong; Luo, Tao; Sheng, Ling; Luo, Yi; Zeng, Yunliu; Xu, Juan; Deng, Xiuxin; Cheng, Yunjiang
• Is Part Of: Plant science. Volume 243(2016:Feb.)
• Page Start: 131
• Page End: 144
• Publication Date: 2016-02
• Subjects: Citrus fruit -- Cutin -- Waxes -- Fruit development -- Metabolic profiling -- Transcript analysis -- ABA; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.12.010
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Cutin was formed before wax accumulation, providing the scaffold for wax assembling. Transition of metabolism of fruits peel occurred from 120 to 150 DAF. ABA and GL1-like were predicted to be pivotal for wax synthesis. Abstract: Fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. However, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. Here, fruit cuticle of 'Newhall' navel orange ( Citrus sinensis Osbeck) was studied from the stage of fruit expansion to postharvest storage from the perspectives of morphology, transcription and metabolism. The results demonstrated that cutin accumulation is synchronous with fruit expansion, while wax synthesis is synchronous with fruit maturation. Metabolic profile of fruits peel revealed that transition of metabolism of fruit peel occurred from 120 to 150 DAF and ABA was predicted to regulate citrus wax synthesis during the development of Newhall fruits. RNA-seq analysis of the peel from the above two stages manifested that the genes involved in photosynthesis were repressed, while the genes involved in the biosynthesis of wax, cutin and lignin were significantly induced at later stages. Further real-time PCR predicted that MYB transcription factor GL1-like regulates citrus fruits wax synthesis. These results are valuable for improving the fruit quality during development and storage.