Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans. (February 2016)

• Record Type: Journal Article
• Authors: Henriquez, Maria Antonia; Soliman, Atta; Li, Genyi; Hannoufa, Abdelali; Ayele, Belay T.; Daayf, Fouad
• Is Part Of: Plant science. Volume 243(2016:Feb.)
• Page Start: 71
• Page End: 83
• Publication Date: 2016-02
• Subjects: Solanum -- DXS -- Phytophthora infestans -- DOXP-MEP -- Jasmonic acid -- Salicylic acid -- Abscisic acid -- Carotenoids -- Terpenoids -- Functional analysis; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.12.001
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: DXS expression varies in response to late blight. Accumulation of isoprenoids is correlated with DXS expression. DXS targets the chloroplast envelops. DXS expression has a negative effect on ABA and GA. Abstract: 1-Deoxy-d -xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2 C -methyl-d -erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1 . StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA contents. Selected downstream genes in the DOXP-MEP pathway, especially GGPPS genes, were up-regulated in the transgenic lines.