Atopic dermatitis-like skin lesions with IgE hyperproduction and pruritus in KFRS4/Kyo rats. Issue 2 (November 2015)

• Record Type: Journal Article
• Authors: Kuramoto, Takashi; Yokoe, Mayuko; Tanaka, Daisuke; Yuri, Azusa; Nishitani, Ai; Higuchi, Yuki; Yoshimi, Kazuto; Tanaka, Miyuu; Kuwamura, Mitsuru; Hiai, Hiroshi; Kabashima, Kenji; Serikawa, Tadao
• Is Part Of: Journal of dermatological science. Volume 80:Issue 2(2015:Nov.)
• Page Start: 116
• Page End: 123
• Publication Date: 2015-11
• Subjects: AD atopic dermatitis -- HE hematoxylin–eosin -- KFRS4 Kyoto Fancy Rat Stock 4 -- LN lymph node -- TB toluidine blue -- TEWL transepidermal water loss -- TSLSS total skin lesion severity score; Atopic dermatitis -- Betamethasone -- IgE -- Pruritus -- Rats; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2015.09.005
• Languages: English
• ISSNs: 0923-1811

Abstract

Highlights: A rat model of atopic dermatitis, Kyoto Fancy Rat Stock 4, has been developed. KFRS4 rats showed spontaneous dermatitis that accompanied scratching behavior. KFRS4 rats exhibited skin barrier dysfunction and elevated IgE levels. Skin lesions were suppressed by topical application of betamethasone. KFRS4 rats are considered to be a good model of atopic dermatitis. Abstract: Background: Rats showing spontaneous atopic dermatitis (AD)-like skin lesions were observed in the Kyoto Fancy Rat Stock 4 (KFRS4) strain breeding colony. Objective: To establish the KFRS4 rat as a model of AD. Methods: The clinical symptoms of AD-like skin lesions were assessed by scoring the degree of dermatitis and examining scratching behavior. The transepidermal water loss was measured to evaluate skin barrier function. Cells infiltrating the skin lesions were identified using histological and immunohistological analyses. IgE and cytokine levels were measured to examine immune status. An ointment treatment experiment was carried out to characterize dermatitis in the KFRS4 rats. Results: Dermatitis initially appeared around 4 months of age and rapidly worsened from 6 to 8 months of age. The skin lesions accompanied scratching behavior and were predominantly observed in females. The increased transepidermal water loss indicated skin barrier dysfunction. Extensive infiltration of eosinophils, mast cells and lymphocytes was observed in the skin lesions. The plasma IgE level increased in accord with increasing severity of dermatitis. The Th2 and Th17 cytokine mRNA levels were significantly higher in the skin-draining lymph nodes than those in the non-skin-draining lymph nodes. It was demonstrated that betamethasone improved the symptoms of dermatitis. These findings demonstrated that dermatitis in the KFRS4 rats closely resembled that seen in human AD. Conclusion: Female KFRS4 rats have the potential to serve as an animal model of human AD.