Soret band shape indicates mTHPC distribution between β-cyclodextrins and serum proteins. (February 2017)

• Record Type: Journal Article
• Authors: Yakavets, Ilya; Yankovsky, Igor; Bezdetnaya, Lina; Zorin, Vladimir
• Is Part Of: Dyes and pigments. Volume 137(2017)
• Page Start: 299
• Page End: 306
• Publication Date: 2017-02
• Subjects: Soret band -- mTHPC -- β-Cyclodextrins -- Serum proteins -- Liposomes; PDT photodynamic therapy -- PS photosensitizer -- mTHPC 5, 10, 15, 20-Tetra(m-hydroxyphenyl)chlorin -- β-CD β-cyclodextrin -- DPPC dipalmitoyl phosphatidyl choline -- DPPG dipalmitoylphosphatidylglycerol -- Me-β-CD methyl-β-cyclodextrin -- TM-β-CD trimethyl-β-cyclodextrin -- DPBS dulbecco's phosphate-buffered saline -- TGF tetrahydrofuran -- φf fluorescence quantum yields -- P fluorescence polarization degree -- r2 adjusted coefficient of determination -- N the fraction of mTHPC molecules bound to Me-β-CD; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2016.11.007
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Fluorescence and absorbance characteristics of 5, 10, 15, 20-tetra( m -hydroxyphenyl)chlorin (mTHPC) in several organic solutions and in complexes with various biological structures have been analyzed. A comparison of data showed that the most variable mTHPC spectral characteristic is the Soret band shape. The ratio of two Soret band peaks are strongly dependent on the kind of biological structure bound with the pigment molecule. It has been suggested, that the observed variations of Soret band shape are associated with conformational changes of mTHPC molecule, caused by the forced rotation of the aryl-substitutes. On the basis of Soret band shape variations, the spectral technique for noninvasive monitoring of mTHPC release from inclusion complex with β-cyclodextrin carriers in blood serum was developed. Graphical abstract: Highlights: The significant difference in Soret band shape was observed for mTHPC molecules bound to β-CD or to serum protein/liposome. The Soret band variations are associated with conformational changes of mTHPC molecule bound to serum protein/liposome/β-CD. The spectral technique for monitoring of mTHPC release from inclusion complex with β-CDs in biological systems was proposed.