Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells. (15th March 2016)

• Record Type: Journal Article
• Authors: Ren, Daoyuan; Wang, Ning; Guo, Jianjun; Yuan, Li; Yang, Xingbin
• Is Part Of: Carbohydrate polymers. Volume 138(2016)
• Page Start: 123
• Page End: 133
• Publication Date: 2016-03-15
• Subjects: Pleurotus eryngii -- Polysaccharide -- Purification -- Anti-proliferation -- Cell cycle -- Apoptosis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.11.051
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: PEP is the crude heteropolysaccharides isolated from Pleurotus eryngii . PEP-1 and PEP-2 were purified from PEP by Cellulos e DEAE-52 and Sephadex G-100. High molecular mass PEP-2 exhibits more potent anticancer effect than PEP-1. ROS was a critical mediator in PEP-2 induced HepG-2 cellular apoptosis. PEP-2 is a good candidate for dietary supplement and therapeutic application in cancer. Abstract: This study was designed to investigate the chemical characterization and antitumor effects of Pleurotus eryngii polysaccharides (PEP). The crude PEP was fractionated into two fractions, namely PEP-1 and PEP-2. HPLC analysis showed that PEP-1 and PEP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 2.54 × 10 4 Da (PEP-1) and 4.63 × 10 5 Da (PEP-2), respectively. High molecular mass PEP-2 was shown to exhibit stronger growth inhibition against human hepatoblastoma HepG-2 cells in comparison with PEP-1. Flow cytometric analysis showed that PEP-2 exerted a stimulatory effect on apoptosis of HepG-2 cells, and induced the cell-cycle arrest at the S-phase, with the observation of intracellular ROS production. These findings suggest that the polysaccharides, especially PEP-2, are very important nutritional ingredients responsible for the anticancer health benefits of P. eryngii .