Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles. (20th January 2016)

• Record Type: Journal Article
• Authors: Bondarenko, Olesja M.; Ivask, Angela; Kahru, Anne; Vija, Heiki; Titma, Tiina; Visnapuu, Meeri; Joost, Urmas; Pudova, Ksenia; Adamberg, Signe; Visnapuu, Triinu; Alamäe, Tiina
• Is Part Of: Carbohydrate polymers. Volume 136(2016)
• Page Start: 710
• Page End: 720
• Publication Date: 2016-01-20
• Subjects: Levan N (PubChem CID: 440946) -- Selenium (PubChem CID: 6326970) -- Cobalt tetraoxide (PubChem CID: 6432046) -- Magnetic oxide (PubChem CID: 14789); Safe-by-design -- Food supplement -- Co, Fe and Se nanomaterial -- Caco-2 in vitro -- Bacteroides thetaiotaomicron; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.09.093
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Levan served as a multifunctional coating material for Fe-, Co- and Se-based NPs. Levan coated NPs and stabilized their dispersion as shown by TEM, DLS and XPS. Levan improved metabolism and stabilized membranes of human cells Caco-2 in vitro. Levan decreased the adverse effects of Co- and Se-based NPs to Caco-2 in vitro. A common colon bacterium Bacteroides thetaiotaomicron solubilized levan-coated cobalt NPs. Abstract: Levan, fructose-composed biopolymer of bacterial origin, has potential in biotechnology due to its prebiotic and immunostimulatory properties. In this study levan synthesized by levansucrase from Pseudomonas syringae was thoroughly characterized and used as multifunctional biocompatible coating material for microelement-nanoparticles (NPs) of selenium, iron and cobalt. Transmission electron microscopy (TEM), hydrodynamic size measurements (DLS) and X-ray photoelectron spectroscopy (XPS) showed the interaction of levan with NPs. Levan stabilized the dispersions of NPs, decreased their toxicity and had protective effect on human intestinal cells Caco-2. In addition, levan attached to cobalt NPs remained accessible as a substrate for the colon bacteria Bacteroides thetaiotaomicron . We suggest that the combination of levan and nutritionally important microelements in the form of NPs serves as a first step towards a novel "2 in 1" approach for food supplements to provide safe and efficient delivery of microelements for humans and support beneficial gut microbiota with nutritional oligosaccharides.