Characterization and immunoenhancement activities of Eucommia ulmoides polysaccharides. (20th January 2016)

• Record Type: Journal Article
• Authors: Feng, Haibo; Fan, Jing; Song, Zhenhui; Du, Xiaogang; Chen, Ying; Wang, Jishuang; Song, Guodong
• Is Part Of: Carbohydrate polymers. Volume 136(2016)
• Page Start: 803
• Page End: 811
• Publication Date: 2016-01-20
• Subjects: Eucommia ulmoides Oliv. polysaccharide -- Adjuvant -- Lymphocyte proliferation -- Cytokines -- Dendritic cell; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.09.079
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Chemical properties, monosaccharide composition, scanning electron microscopy (SEM) analyses, and immunoenhancement activities of EUPS were firstly characterized. EUPS significantly stimulated proliferation of splenic lymphocyte in vitro . EUPS significantly promoted IL-4 and IFN-γ production of T lymphocyte in vitro . EUPS could activate DCs and induce them to the maturation in vitro . EUPS as an adjuvant significantly improved the humoral and cellular immune response in vivo . Abstract: The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv . polysaccharide. The average molecular weight (Mw) of EUPS was 11.4632 × 10 5 Da. The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively. In in vitro experiments, EUPS (1.2–75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation. Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production. In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation. Together, these results suggest that EUPS is a strong immunostimulant.