Crystal Structure of Human General Transcription Factor TFIIE at Atomic Resolution. Issue 21 (23rd October 2016)

• Record Type: Journal Article
• Authors: Miwa, Kohei; Kojima, Rieko; Obita, Takayuki; Ohkuma, Yoshiaki; Tamura, Yasushi; Mizuguchi, Mineyuki
• Is Part Of: Journal of molecular biology. Volume 428:Issue 21(2016:Oct. 23)
• Page Start: 4258
• Page End: 4266
• Publication Date: 2016-10-23
• Subjects: GTF general transcription factor -- Pol II RNA polymerase II -- PIC pre-initiation complex -- EM electron microscopy -- TBP TATA box-binding protein -- WH winged helix -- ZnF zinc-finger -- CC coiled-coil -- bH basic helix -- ITC isothermal titration calorimetry -- LD lipoyl domain -- 5′-fluoroorotic acid 5′-fluoroorotic acid -- sso Sulfolobus solfataricus -- dsDNA double-stranded DNA; TFIIEα/β -- RNA polymerase II -- transcription -- yeast -- pre-initiation complex; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2016.09.008
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: In eukaryotes, RNA polymerase II requires general transcription factors to initiate mRNA transcription. TFIIE subunits α and β form a heterodimer and recruit TFIIH to complete the assembly of the pre-initiation complex. Here, we have determined the crystal structure of human TFIIE at atomic resolution. The N-terminal half of TFIIEα forms an extended winged helix (WH) domain with an additional helix, followed by a zinc-finger domain. TFIIEβ contains the WH2 domain, followed by two coiled-coil helices intertwining with TFIIEα. We also showed that TFIIEα binds to TFIIEβ with nanomolar affinity using isothermal titration calorimetry. In addition, mutations on the residues involved in the interactions resulted in severe growth defects in yeast. Lack of the C-terminal region of yeast TFIIEβ causes a mild growth defect in vivo . These findings provide a structural basis for understanding the functional mechanisms of TFIIE in the context of pre-initiation complex formation and transcription initiation. Graphical Abstract: Highlights: The crystal structure of the TFIIE complex was determined. TFIIEα adopts an extended WH fold. The residues involved in the interactions between yeast Tfa1 (TFIIEα in human) and Tfa2 (TFIIEβ in human) are essential for yeast cell growth.