The effect of induced hypertension on neurological outcome in forebrain ischaemia model in rats. Issue 3 (March 2016)

• Record Type: Journal Article
• Authors: Jung, Yoo Sun; Han, Ye-Reum; Kim, Byung-Gun; Oh, Ah-Young; Park, Hee-Pyoung; Hwang, Jung-Won; Lim, Young-Jin; Jeon, Young-Tae
• Is Part Of: Injury. Volume 47:Issue 3(2016)
• Page Start: 605
• Page End: 608
• Publication Date: 2016-03
• Subjects: Apoptosis -- Brain ischaemia -- Hypertension -- Necrosis -- Phenylephrine; Wounds and injuries -- Surgery -- Periodicals; Accidents -- Periodicals; Wounds and Injuries -- surgery -- Periodicals; Lésions et blessures -- Chirurgie -- Périodiques; Electronic journals; Electronic journals; 617.1
• DOI: 10.1016/j.injury.2015.11.003
• Languages: English
• ISSNs: 0020-1383

Abstract

Abstract: Introduction: The present study investigated the effects of induced hypertension on hippocampal cell death after forebrain ischaemia in rats. Materials and methods: In this study, forebrain ischaemia was induced in 20 Sprague-Dawley rats by clamping the bilateral common carotid arteries to induce systemic hypotension for 8 min. All rats then underwent reperfusion during which the induced hypertension group ( n = 10) received intermittent intravenous injections of phenylephrine (5 μg) to maintain their mean arterial blood pressure at 20 mmHg above baseline for 10 min and the control group ( n = 10) did not receive any treatment. In both groups, the numbers of viable and apoptotic neuronal cells in the cornu ammonis 1 (CA1) area of the hippocampus were evaluated 7 days after the induction of ischaemia. Results: The mean percentage of viable neuronal cells was higher in the induced hypertension group than in the control group (35% vs. 26%, respectively; p = 0.004), but there was no significant difference in the proportion of apoptotic neuronal cells between the groups (57% vs. 43%, respectively; p = 0.165). Conclusions: Induced hypertension significantly attenuated necrotic cell death in the hippocampal CA1 area, but apoptotic cell death was not affected.