Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1. (2nd September 2015)

• Record Type: Journal Article
• Authors: Van Calsteren, Marie-Rose; Gagnon, Fleur; Nishimura, Junko; Makino, Seiya
• Is Part Of: Carbohydrate research. Volume 413(2015)
• Page Start: 115
• Page End: 122
• Publication Date: 2015-09-02
• Subjects: Nuclear magnetic resonance (NMR) -- casper -- Smith degradation -- Mass spectrometry (MS) -- Repeating unit sequence; 1D one-dimensional -- 2D two-dimensional -- APS anionic polysaccharide -- CHORTLE carbon–hydrogen correlations from one-dimensional polarization-transfer spectra by least-squares analysis -- CID collision-induced dissociation -- COSY correlation spectroscopy -- DMSO dimethylsulfoxide -- DSS 2, 2-dimethyl-2-silapentane-5-sulfonate -- EPS exopolysaccharide -- ESI electrospray ionization -- FID flame-ionization detection -- GLC gas–liquid chromatography -- HETCOR heteronuclear correlation -- HMBC heteronuclear multiple-bond correlation -- HSQC heteronuclear single-quantum coherence -- MALS multi-angle light scattering -- MS mass spectrometry -- NK natural killer -- NMR nuclear magnetic resonance -- NOESY nuclear Overhauser effect spectroscopy -- NPS neutral polysaccharide -- PMAA partially methylated alditol acetate -- RELAY relayed COSY -- RI refractive index -- RMS root mean square -- ROESY rotating-frame Overhauser effect spectroscopy -- SEC size-exclusion chromatography -- TCA trichloroacetic acid -- TFA trifluoroacetic acid -- TOCSY total correlation spectroscopy -- UV ultraviolet; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2015.05.015
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp. bulgaricus strain OLL1073R-1 was purified and characterized. The molecular mass was 5.0×10 6 g/mol. Sugar and absolute configuration analyses gave the following composition:d -Glc, 1;d -Gal, 1.5. The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analyses, 1 H and 13 C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1–3)Glc(β1–3)[Gal(β1–4)]Gal(β1–4)Gal(α1–} n . The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains. Graphical abstract: Highlights: Strain OLL1073R-1 produces a high-molecular-weight exopolysaccharide. The repeating unit is a branched pentasaccharide composed of Glc and Gal. The structure is compared to that of other strains of the same species.