Structure of the O-specific polysaccharide from the deep-sea marine bacterium Idiomarina abyssalis КММ 227T containing a 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3, 6-dideoxy-d-glucose. (2nd September 2015)

• Record Type: Journal Article
• Authors: Kokoulin, Maxim S.; Komandrova, Nadezhda A.; Kalinovskiy, Anatoly I.; Tomshich, Svetlana V.; Romanenko, Lyudmila A.; Vaskovsky, Victor V.
• Is Part Of: Carbohydrate research. Volume 413(2015)
• Page Start: 100
• Page End: 106
• Publication Date: 2015-09-02
• Subjects: Extremophiles -- Marine bacterium -- Idiomarina abyssalis -- O-Specific polysaccharide -- Sulfate -- 2-O-Sulfate-3-N-(4-hydroxybutanoyl)-3, 6-dideoxy-d-glucose; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2015.05.012
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The O-specific polysaccharide was isolated from the lipopolysaccharide of type strain Idiomarina abyssalis КММ 227 T and studied by sugar analysis, Smith degradation, and two-dimensional 1 H and 13 C NMR spectroscopy including 1 H, 1 H-TOCSY, 1 H, 1 H-COSY, 1 H, 1 H-ROESY, 1 H, 13 C-HSQC, 1 H, 13 C-HMBC, 1 H, 13 C-H2BC and 1 H, 13 C-HSQC-TOCSY experiments. The new structure of the O-specific polysaccharide of I. abyssalis КММ 227 T containing 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3, 6-dideoxy-d -glucose was established: Graphical abstract: Highlights: Structure of the O-polysaccharide of Idiomarina abyssalis КММ 227 T was established. The O-polysaccharide is built up of sulfated pentasaccharide repeating units. The O-polysaccharide contains 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3, 6-dideoxy-d -glucose.