Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits. (5th November 2015)

• Record Type: Journal Article
• Authors: Yu, Zeyuan; Liu, Lu; Xu, Yaqin; Wang, Libo; Teng, Xin; Li, Xingguo; Dai, Jing
• Is Part Of: Carbohydrate polymers. Volume 132(2015)
• Page Start: 180
• Page End: 186
• Publication Date: 2015-11-05
• Subjects: Rubus idaeus L. -- Polysaccharide -- Purification -- Antioxidant activity -- Non-enzymatic glycation inhibition activity; d-Glucose (PubChem CID: 5793) -- d-Arabinose (PubChem CID: 66308) -- d-Galacturonic acid (PubChem CID: 84740) -- Trifluoroacetic acid (PubChem CID: 6422) -- Ascorbic acid (PubChem CID: 54670067) -- Carbazole (PubChem CID: 6854) -- Girard-T (PubChem CID: 67156) -- DPPH (PubChem CID: 2735032); Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.06.068
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A novel water-soluble polysaccharide namely RCP-II was obtained from raspberry. Preliminary characterization of RCP-II was performed by GC, HPLC, IR and NMR. RCP-II exhibited certain antioxidant capability and non-enzymatic glycation inhibition activity. Abstract: A water-soluble polysaccharide namely RCP-II from raspberry fruits was obtained by complex enzyme method followed by successive purification using macroporous resin D4020 and Sephadex G-100 columns. RCP-II was an acidic heteropolysaccharide and the characteristic structure of polysaccharide was determined. The carbohydrate of RCP-II was composed with galacturonic acid, rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.00:0.55:1.19:0.52:0.44:1.90 and the average molecular weight was estimated to be 4013 Da, based on dextran standards. RCP-II presented high scavenging activity toward DPPH, HO, O2 − in a concentration-dependent manner. The determination of the inhibitory activity on protein glycation showed that in 14 days of incubation the inhibitory ability of RCP-II was more effective on the development of non-enzymatic glycation reaction at early phase than that at the following two phases.