Optimization of polysaccharides extraction from Tricholoma mongolicum Imai and their antioxidant and antiproliferative activities. (20th October 2015)

• Record Type: Journal Article
• Authors: Wang, Jin; Zhao, Yongming; Li, Wei; Wang, Zhibao; Shen, Lixia
• Is Part Of: Carbohydrate polymers. Volume 131(2015)
• Page Start: 322
• Page End: 330
• Publication Date: 2015-10-20
• Subjects: Tricholoma mongolicum Imai polysaccharides -- Enzyme-assisted extraction -- Response surface methodology -- Antioxidant activity -- Antiproliferative activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.06.009
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Enzyme-assisted extraction for Tricholoma mongolicum Imai polysaccharides was built. Enzyme amount, extraction time, pH and temperature were optimized by a Box–Behnken design. Four polysaccharides were purified and characterized. Polysaccharides exhibited potent antioxidant and antiproliferative activities. Abstract: Response surface methodology was used to optimize the enzyme-assisted extraction parameters for polysaccharides from Tricholoma mongolicum Imai (TMIPs). The orthogonal test design was employed to determine the optimal concentration of three kinds of enzyme (trypsin, pectinase and papain) and the optimal concentrations of trypsin, pectinase and papain were 1.5%, 2.5%, and 2.0% (dry weight % of T. mongolicum Imai powder), respectively. In addition, three variables that remarkably affected the yield of polysaccharides such as extraction temperature, pH and extraction time were studied based on a Box–Behnken design. The results demonstrated that extraction time was the most remarkable factor affecting the TMIPs yield, followed by pH and temperature. Optimal extraction was obtained at 48.4 °C, pH 5.4, and extraction time of 132 min. Under these optimum conditions, the yield was 24.01%, which is consistent with the predicted value. Furthermore, crude polysaccharides were purified to obtain four fractions. In vitro antioxidant and antiproliferative activities results showed that TMIP-4 had stronger antioxidant and antiproliferative capacity than other fractions.