Development of bio-composites with novel characteristics: Evaluation of phenol-induced antibacterial, biocompatible and biodegradable behaviours. (20th October 2015)

• Record Type: Journal Article
• Authors: Iqbal, Hafiz M.N.; Kyazze, Godfrey; Locke, Ian C.; Tron, Thierry; Keshavarz, Tajalli
• Is Part Of: Carbohydrate polymers. Volume 131(2015)
• Page Start: 197
• Page End: 207
• Publication Date: 2015-10-20
• Subjects: Laccase -- Bio-composite, Gallic acid -- Thymol -- Antibacterial -- HaCaT compatible; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.05.046
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Enzymatic biotransformation of gallic acid, thymol, P(3HB) and EC into value-added graft material. Antibacterial bio-composites as potential candidates for biomedical applications. Biocompatible and biodegradable laccase-assisted bio-composites for bio-sector. Abstract: This paper describes a laccase-assisted grafting of gallic acid (GA) and thymol (T) as functional entities onto the previously developed P(3HB)- g -EC composite. GA- g -P(3HB)- g -EC and T- g -P(3HB)- g -EC bio-composites were prepared by laccase-assisted free radical-induced graft polymerisation of GA and T onto the P(3HB)- g -EC based composite using surface dipping and incorporation technique. The results of the antibacterial evaluation for the prepared composites indicated that 15GA- g -P(3HB)- g -EC, 15T- g -P(3HB)- g -EC and 20T- g -P(3HB)- g -EC composites possessed the strongest bacteriostatic and bactericidal activities against Gram-positive Bacillus subtilis NCTC 3610 and Staphylococcus aureus NCTC 6571 and Gram-negative Escherichia coli NTCT 10418 and Pseudomonas aeruginosa NCTC 10662 strains. In this study, we have also tested GA- g -P(3HB)- g -EC and T- g -P(3HB)- g -EC bio-composites for their ability to support and maintain multilineage differentiation of human keratinocyte-like (HaCaT) skin cells in-vitro . From the cytotoxicity results, the tested composites showed 100% viability and did not induce any adverse effect on a HaCaT's morphology. Finally, in soil burial evaluation, a progressive increase in the degradation rate of GA- g -P(3HB)- g -EC and T- g -P(3HB)- g -EC bio-composites was recorded with the passage of time up to 6 weeks. In summary, our current findings suggest that GA- g -P(3HB)- g -EC and T- g -P(3HB)- g -EC bio-composites are promising candidates for biomedical type applications such as skin regeneration, multiphasic tissue engineering and/or medical implants.