Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro. (20th October 2015)

• Record Type: Journal Article
• Authors: Chen, Qixin; Mei, Xifan; Han, Guanying; Ling, Peixue; Guo, Bin; Guo, Yuewei; Shao, Huarong; Wang, Guan; Cui, Zan; Bai, Yuxin; Xu, Fang
• Is Part Of: Carbohydrate polymers. Volume 131(2015)
• Page Start: 363
• Page End: 369
• Publication Date: 2015-10-20
• Subjects: Xanthan gum (PubChem CID: 7107) -- Sodium hyaluronate (PubChem CID: 3084049) -- Nitroprusside (PubChem CID: 11963622) -- 3, 5-Diphenyl-1-(4, 5-dimethyl-2-thiazolyl) formazan (PubChem CID: 3634887) -- Rhodamin 123 (PubChem CID: 9929799) -- Propidium iodide (PubChem CID: 104981); Xanthan gum -- Sodium nitroprusside -- Rabbit chondrocytes -- Apoptosis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.06.004
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Xanthan gum reversed SNP-reduced rabbit chondrocytes proliferation. Xanthan gum declined early apoptosis rate and maintained the morphology of SNP-induced rabbit chondrocytes. Xanthan gum alleviated SNP-induced rabbit chondrocytes loss/disruption of mitochondrial membrane potential. Xanthan gum down-regulated the levels of PGE2 in SNP-induced rabbit chondrocytes in medium. Abstract: We have previously reported that intra-articular injection of xanthan gum (XG) could significantly ameliorate the degree of joint cartilage degradation and pain in experimental osteoarthritis (OA) model in vivo . In this present study, we evaluated the protective effect of XG against Sodium nitroprusside (SNP)-induced rabbit articular chondrocytes apoptosis in vitro . Rabbit articular chondrocytes were incubated with various concentrations of XG for 24 h prior to 0.5 mmol/L SNP co-treatment for 24 h. The proliferation of chondrocytes was analyzed using MTT assay. The chondrocytes early apoptosis rates were evaluated using Annexin V-FITC/PI flow cytometry. The morphology of apoptosis chondrocytes were observed by scanning electron microscopy (SEM). The loss/disruption of mitochondrial membrane potential was detected using rhodamin 123 by confocal microscope. The concentration of prostaglandin E2 (PGE2 ) in cell culture supernatants was evaluated using ELISA assay. The results showed that XG could significantly reverse SNP-reduced cell proliferation and inhibited cell early apoptosis rate in a dose-dependent manner. XG alleviated loss/disruption of mitochondrial membrane potential and decreased the PGE2 level of chondrocytes cell culture supernatants in SNP-induced chondrocytes. These results of the present research strongly suggest that XG can protect rabbit articular chondrocytes against SNP-induced apoptosis in vitro .