Human Amniotic Membrane Is Not Suitable for the Grafting of Colon Lesions and Prevention of Adhesions in a Xenograft Rat Model. (August 2017)

• Record Type: Journal Article
• Authors: Barski, Dimitri; Gerullis, Holger; Ecke, Thorsten; Varga, Gabriella; Boros, Mihaly; Pintelon, Isabel; Timmermans, Jean-Pierre; Otto, Thomas
• Is Part Of: Surgical innovation. Volume 24:Number 4(2017:Aug.)
• Page Start: 313
• Page End: 320
• Publication Date: 2017-08
• Subjects: amniotic membrane -- xenolog transplantation -- bowel lesion -- anastomosis -- adhesions -- IDEAL recommendations; Surgery, Operative -- Periodicals; Endoscopic surgery -- Periodicals; Laparoscopic surgery -- Periodicals; Surgical Procedures, Operative -- Periodicals; Surgical Procedures, Minimally Invasive -- Periodicals; Diffusion of Innovation -- Periodicals; Chirurgie opératoire -- Périodiques; Chirurgie endoscopique -- Périodiques; Chirurgie laparoscopique -- Périodiques; 617.91
• DOI: 10.1177/1553350617709828
• Languages: English
• ISSNs: 1553-3506

Abstract

Introduction . New biological materials are needed for specific applications in reconstructive bowel surgery and for the prevention of adhesion formation. Amniotic membranes (AMs) are assumed to have a number of unique characteristics that enhance the ingrowth of the surrounding tissue. The aim of the present study was to provide proof of these qualities in a xenograft model. Materials and methods . A multilayer human AM (HAM) was applied to repair defined colon wall defects in Sprague-Dawley rats (n = 18). The control group was repaired with a suture (n = 6). The animals were killed humanely at 7, 21, and 42 days after implantation. Adhesions and perioperative complications were examined. Histological and immunohistological analyses were performed to assess a number of parameters, including degradation of the HAM, inflammation, graft rejection, and smooth muscle ingrowth. Results . Two rats in the treated group died. No other severe complications were observed. Adhesion formation was more prominently visible in the HAM group ( P < .05). The initially increased inflammation in the HAM group reduced over time but remained significantly increased ( P < .05). The HAM degraded over time and a subtle transient glomerulitis could be observed. Conclusion . HAMs were found to increase adhesion formation and were not suitable for bowel augmentation in the presented xenograft model.