Expression of Apoptotic vs Antiapoptotic Proteins in Middle Ear Cholesteatoma. (December 2015)

• Record Type: Journal Article
• Authors: Chung, Jae Ho; Lee, Seung Hwan; Park, Chul Won; Kim, Kyung Rae; Tae, Kyung; Kang, Sung-Ho; Oh, Young Ha; Pyo, Ju Yeon
• Is Part Of: Otolaryngology--head and neck surgery. Volume 153:Number 6(2015:Dec.)
• Page Start: 1024
• Page End: 1030
• Publication Date: 2015-12
• Subjects: cholesteatoma -- apoptosis -- p53 -- c-FLIP -- Ki-67; Head -- Surgery -- Periodicals; Neck -- Surgery -- Periodicals; Otolaryngology -- Periodicals; 617.51
• DOI: 10.1177/0194599815591810
• Languages: English
• ISSNs: 0194-5998

Abstract

Objectives: To explore the role of antiapoptotic and apoptotic processes in the development of cholesteatoma by investigating the expression of an antiapoptotic (c-FLIP) and apoptotic (p53) protein relative to the expression of a proliferation marker (Ki-67). Study Design: Basic science study. Setting: Tertiary referral center. Subjects and Methods: An immunohistochemical investigation was performed on 35 cholesteatoma specimens (21 acquired, 14 congenital) and 10 normal retroauricular skins to evaluate the expression of c-FLIP, p53, and Ki-67. The expression rate of each marker was measured to assess the difference between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma. Results: c-FLIP expression was significantly higher in the cholesteatoma specimens than in retroauricular skin ( P < .05), while the expression of p53 did not significantly differ between the two. Ki-67 expression in cholesteatoma was significantly higher than in retroauricular skin ( P < .001). The c-FLIP expression rate was positively correlated with that of Ki-67 ( r = 0.47, P = .001), and there was no significant correlation between the expression level of p53 and that of Ki-67 ( r = 0.152, P = .319). In addition, no differences in c-FLIP, p53, and Ki-67 expression rates were evident between congenital and acquired cholesteatoma. Conclusions: The upregulation of c-FLIP together with unchanged p53 suggests an altered equilibrium between apoptosis and antiapoptosis, favoring antiapoptosis, and may play a role in the pathogenesis of cholesteatoma.