Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model. (October 2018)

• Record Type: Journal Article
• Authors: Bakht azad, Sima; Nikokar, Iraj; Faezi, Sobhan; Rasooly, Soheila; Mahdavi, Mehdi
• Is Part Of: Microbial pathogenesis. Volume 123(2018)
• Page Start: 426
• Page End: 432
• Publication Date: 2018-10
• Subjects: Pseudomonas aeruginosa -- PilQ -- Flagellin -- Burn -- Immunization -- Cytokine; r-PilQ recombinant PilQ -- FLB type b-flagellin -- P. aeruginosa Pseudomonas aeruginosa -- Tfp type IV pili -- PAMP pathogen-associated molecular pattern -- TLR5 Toll like receptor-5 -- LB Luria-Bertani -- OD optical density -- TMB Tetramethylbenzidine -- pen-strep penicillin-streptomycin; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.07.042
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Considering the increased antibiotic resistance of Pseudomonas aeruginosa, the evaluation of immune response against the antigens of this bacterium seems necessary. In this study, the protective efficacy and immunological properties of P. aeruginosa recombinant PilQ (r-PilQ) and type b-flagellin (FLB) proteins was evaluated in the burn mouse model of infection. The inbred BALB/c mice were immunized with r-PilQ and FLB antigens. To investigate the type of induced immune response, sera were analyzed by ELISA for total IgG, IgG1, and IgG2a isotypes. After the final immunization, the IL-4, IFN-γ, and IL-17 cytokines level were examined in the spleen of non-challenged mice. Fifty days after lethal challenge, the survival rate and bacterial burden in the skin and other internal organs of experimental mice were assessed. The in vivo administration of r-PilQ, FLB and combined antigen resulted in a significant increase in the survival of mice (66%, 75%, and 83%, respectively) infected by the PAO1 strain of P. aeruginosa in the burn model of infection. Immunization of mice with r-PilQ and FLB mixture induced high titers of IL-4 and IL-17 cytokines compared to control groups ( P < 0.05). The high titer of antisera raised against combined antigen was able to inhibit the systemic spread of the PAO1 strain from the site of infection to the internal organs. We concluded that the parallel role of IL-4 and IL-17 is necessary for elimination of the bacteria and promotion of survival in the immunized burn mice. Highlights: Administration of combined antigen increased the survival of mice. Combined antigen induced high titers of IL-4 and IL-17 cytokines. The anti-combined antigen antisera inhibited the systemic spread of PAO1 strain.