Assessing the detectability of antioxidants in two‐dimensional high‐performance liquid chromatography. Issue 10 (17th April 2015)

• Record Type: Journal Article
• Authors: Bassanese, Danielle N.; Conlan, Xavier A.; Barnett, Neil W.; Stevenson, Paul G.
• Is Part Of: Journal of separation science. Volume 38:Issue 10(2015:May)
• Page Start: 1642
• Page End: 1648
• Publication Date: 2015-04-17
• Subjects: Antioxidants -- Countergradient -- Limit of detection -- Solvent strength mismatch -- Two‐dimensional high‐performance liquid chromatography; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201500054
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : This paper explores the analytical figures of merit of two‐dimensional high‐performance liquid chromatography for the separation of antioxidant standards. The cumulative two‐dimensional high‐performance liquid chromatography peak area was calculated for 11 antioxidants by two different methods—the areas reported by the control software and by fitting the data with a Gaussian model; these methods were evaluated for precision and sensitivity. Both methods demonstrated excellent precision in regards to retention time in the second dimension (%RSD below 1.16%) and cumulative second dimension peak area (%RSD below 3.73% from the instrument software and 5.87% for the Gaussian method). Combining areas reported by the high‐performance liquid chromatographic control software displayed superior limits of detection, in the order of 1 × 10 −6 M, almost an order of magnitude lower than the Gaussian method for some analytes. The introduction of the countergradient eliminated the strong solvent mismatch between dimensions, leading to a much improved peak shape and better detection limits for quantification.