A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells. Issue 46 (13th November 2017)

• Record Type: Journal Article
• Authors: Wang, Minjiao; Wang, Zhihua; Zhang, Mingkan; Guo, Wei; Li, Ning; Deng, Yuliang; Shi, Qihui
• Is Part Of: Journal of materials chemistry. Volume 5:Issue 46(2017)
• Page Start: 9114
• Page End: 9120
• Publication Date: 2017-11-13
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c7tb02318a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : This paper reports an analytical model to geometrically optimize a herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency. Abstract : A microfluidic chip with single-sided herringbone microstructure has been developed to isolate circulating tumor cells (CTCs) from blood samples of cancer patients. Here, we describe a new double-sided herringbone chip in which staggered herringbone micromixers are placed on both top and bottom surfaces of microchannels. The double-sided herringbone structure enables a high CTC capture efficiency of whole blood samples without depletion of red blood cells because of the effects of leukocyte margination and plasma skimming. However, compared with the traditional single-sided herringbone chip, the double-sided herringbone chip has more complicated geometrical design, leading to a difficulty in experimental optimization of geometrical parameters. In this study, we developed an analytical model to geometrically optimize the herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency. On-chip cell capture experiments for validating modeling results were performed by spiking cultured EpCAM-positive tumor cells into blood samples from healthy donors. Based on the geometrical parameters optimized from the single-sided herringbone chip, the geometrically optimized double-sided herringbone chip enables a capture efficiency of 94 ± 4% of rare tumor cells directly from whole blood.